Detection of Ehrlichia Risticii using an Avidin-Biotin-Immunoperoxidase Staining System
نویسندگان
چکیده
منابع مشابه
Amplified detection of avidin-biotin binding using terahertz wave technology
A new affinity biosensor based on a novel terahertz (THz) wave technology, differential time domain terahertz spectroscopy (DTDS), has been used to monitor the binding between biotin and avidin molecules. Amplified detection of ligandreceptor binding on supported membranes composed of a biotin bilayer on quartz modified octadecanol surface is a useful model lipid membrane for studying avidinbio...
متن کاملSolid phase DNA sequencing using the biotin-avidin system.
A novel method for solid-phase DNA sequencing is described. A plasmid vector, pRIT27, has been designed to allow directional immobilization of double stranded plasmid to avidin agarose. The strategy involves enzymatic incorporation of 11-bio-dUTP into the plasmid and strand specific elution using alkali. The immobilized single stranded DNA is used as template for sequencing reactions and the re...
متن کاملImmunoperoxidase staining of leptospires.
Leptospires were detectable by light microscopy when Formalin-fixed preparations were reacted by direct and indirect methods with horseradish peroxidase-labeled specific antibody.
متن کاملUnbinding biotin from avidin
Atomic force microscopy of single molecules, steered molecular dynamics and the theory of stochastic processes have established a new field that investigates mechanical functions of proteins, such as ligand–receptor binding/unbinding and elasticity of muscle proteins during stretching. The combination of these methods yields information on the energy landscape that controls mechanical function ...
متن کاملUse of the avidin-biotin complex for specific staining of biological membranes in electron microscopy.
To expand the electron microscopist's options in localization and visualization, a new and general staining technique has been tested. The avidin-biotin complex serves as a coupling between the electron-dense marker, ferritin, and points of interest in biological samples. When specific cellular components are tagged with biotin, those components may be visualized with ferritin-linked avidin. Be...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1989
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063878900100303